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Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

14,902 Views

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07:52 min

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July 23, 2012

DOI:

10.3791/4014-v

DOI:
10.3791/4014-v

•

07:52 min
July 23, 2012

•

2 Views
,
1Stem Cell and Cancer Research Institute,McMaster University, Hamilton, 2Physiology and Experimental Medicine Research Program, Hospital for Sick Children,University of Toronto

Summary

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We describe a strategy to monitor maturation and migration of pulmonary dendritic cells in response to ovalbumin in the setting of ovalbumin induced allergic airway inflammation. This strategy can be modified to assess migration of pulmonary dendritic cells in settings of infection.

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Pulmonary Dendritic CellsMaturationMigrationAllergic Airway InflammationAdaptive Immune ResponseAntigen-specific T CellsAntigen StimulationDraining Lymph NodesT Cell ResponseAutoimmune ResponseAllergic Immune ResponseImmune ToleranceLung EnvironmentEnvironmental AntigensAllergyAsthmaAirway DC MaturationAirway DC MigrationSensitizationOvalbumin-induced Allergic Asthma

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Cite this Article

Kushwah, R., Hu, J. Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation. J. Vis. Exp. (65), e4014, doi:10.3791/4014 (2012).

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