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Imunologia e Infecção
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Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation

DOI:
10.3791/4014-v

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07:52 min

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July 23, 2012

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,
1Stem Cell and Cancer Research Institute,McMaster University, Hamilton, 2Physiology and Experimental Medicine Research Program, Hospital for Sick Children,University of Toronto

Capítulos

  • 00:05Título
  • 01:24Sensitizing and Intranasal Challenge of Mice with Ovalbumin
  • 02:39Preparation of Single-cell Suspension from the Lungs and the Draining Lymph Nodes
  • 05:37Results: OVA Sensitization and Challenge Induces an Increase in CD11c+ Cell Trafficking to Murine Mediastinal Lymph Nodes
  • 07:33Conclusion

Summary

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We describe a strategy to monitor maturation and migration of pulmonary dendritic cells in response to ovalbumin in the setting of ovalbumin induced allergic airway inflammation. This strategy can be modified to assess migration of pulmonary dendritic cells in settings of infection.

Tags

Pulmonary Dendritic CellsMaturationMigrationAllergic Airway InflammationAdaptive Immune ResponseAntigen-specific T CellsAntigen StimulationDraining Lymph NodesT Cell ResponseAutoimmune ResponseAllergic Immune ResponseImmune ToleranceLung EnvironmentEnvironmental AntigensAllergyAsthmaAirway DC MaturationAirway DC MigrationSensitizationOvalbumin-induced Allergic Asthma

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