Journal
/
신경과학
/
Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

DOI:
10.3791/4008-v

•

06:47 min

•

April 19, 2012

•

,
1Developmental Neurobiology Section,National Institute of Neurological Disorders and Stroke, National Institutes of Health

Chapters

  • 00:05Title
  • 01:44Pipette Preparation and Positioning
  • 02:40Dye Application
  • 04:36Incubation
  • 04:56Results: Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
  • 05:59Conclusion

Summary

자동 번역

자동 번역

We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.

Tags

Retrograde LabelingNervesTractsSpinal RootsFluorescent DyesCalcium-sensitive DyesVoltage-sensitive DyesSolid CrystalsLocal Pressure InjectionGlass PipettesDye DiffusionPolyethylene Suction Pipette

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves

A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Read Article