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Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
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Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

12,118 Views

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06:47 min

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April 19, 2012

DOI:

10.3791/4008-v

DOI:
10.3791/4008-v

•

06:47 min
April 19, 2012

•

2 Views
,
1Developmental Neurobiology Section,National Institute of Neurological Disorders and Stroke, National Institutes of Health

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We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.

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Retrograde LabelingNervesTractsSpinal RootsFluorescent DyesCalcium-sensitive DyesVoltage-sensitive DyesSolid CrystalsLocal Pressure InjectionGlass PipettesDye DiffusionPolyethylene Suction Pipette

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Blivis, D., O'Donovan, M. J. Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes. J. Vis. Exp. (62), e4008, doi:10.3791/4008 (2012).

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