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Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
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Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
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April 19, 2012
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We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.
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Blivis, D., O'Donovan, M. J. Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes. J. Vis. Exp. (62), e4008, doi:10.3791/4008 (2012).
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