ジャーナル
/
神経科学
/
Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

DOI:
10.3791/4008-v

•

06:47 min

•

April 19, 2012

•

,
1Developmental Neurobiology Section,National Institute of Neurological Disorders and Stroke, National Institutes of Health

章

  • 00:05標題
  • 01:44Pipette Preparation and Positioning
  • 02:40Dye Application
  • 04:36Incubation
  • 04:56Results: Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
  • 05:59Conclusion

概要

自動翻訳

自動翻訳

We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.

タグ

Retrograde LabelingNervesTractsSpinal RootsFluorescent DyesCalcium-sensitive DyesVoltage-sensitive DyesSolid CrystalsLocal Pressure InjectionGlass PipettesDye DiffusionPolyethylene Suction Pipette

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves

A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Read Article