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Competitive Genomic Screens of Barcoded Yeast Libraries
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August 11, 2011
DOI:
1Banting and Best Department of Medical Research and Department of Molecular Genetics,University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, 3Donnelly Sequencing Centre,University of Toronto, 4Genetics and Molecular Biology Branch,National Human Genome Research Institute, NIH, 5Stanford Genome Technology Center, Stanford School of Medicine,Stanford University , 6Department of Pharmaceutical Sciences,University of Toronto
Summary
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We have developed comprehensive, unbiased genome-wide screens to understand gene-drug and gene-environment interactions. Methods for screening these mutant collections are presented.
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Smith, A. M., Durbic, T., Oh, J., Urbanus, M., Proctor, M., Heisler, L. E., Giaever, G., Nislow, C. Competitive Genomic Screens of Barcoded Yeast Libraries. J. Vis. Exp. (54), e2864, doi:10.3791/2864 (2011).
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