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Competitive Genomic Screens of Barcoded Yeast Libraries
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

DOI:

11:59 min

August 11, 2011

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Capítulos

  • 00:05Título
  • 01:55Combining Individual Mutants into a Single Pool
  • 03:23Experimental Pool Growth
  • 04:56Genomic DNA Extraction, PCR and Microarray Hybridization
  • 08:33Sequencing Barcoded Yeast Strains
  • 09:22Genomic Screening Results
  • 11:03Conclusion

Summary

Traducción Automática

We have developed comprehensive, unbiased genome-wide screens to understand gene-drug and gene-environment interactions. Methods for screening these mutant collections are presented.

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