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Competitive Genomic Screens of Barcoded Yeast Libraries
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

DOI:
10.3791/2864-v

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11:59 min

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August 11, 2011

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, , , , , , ,
1Banting and Best Department of Medical Research and Department of Molecular Genetics,University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, 3Donnelly Sequencing Centre,University of Toronto, 4Genetics and Molecular Biology Branch,National Human Genome Research Institute, NIH, 5Stanford Genome Technology Center, Stanford School of Medicine,Stanford University , 6Department of Pharmaceutical Sciences,University of Toronto

Chapters

  • 00:05Title
  • 01:55Combining Individual Mutants into a Single Pool
  • 03:23Experimental Pool Growth
  • 04:56Genomic DNA Extraction, PCR and Microarray Hybridization
  • 08:33Sequencing Barcoded Yeast Strains
  • 09:22Genomic Screening Results
  • 11:03Conclusion

Summary

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We have developed comprehensive, unbiased genome-wide screens to understand gene-drug and gene-environment interactions. Methods for screening these mutant collections are presented.

Tags

Competitive Genomic ScreensBarcoded Yeast LibrariesNext Generation SequencingGenome SequencesGene FunctionDeletion MutantsHigh-throughput PhenotypingDNA TagsBarcodesMutant FitnessTransposon Disruption-barcoding ApproachCandida Albicans Barcoded Disruption

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