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酸修饰的寡核苷酸锁定核酸转染和蚊虫细胞的靶基因突变
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
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酸修饰的寡核苷酸锁定核酸转染和蚊虫细胞的靶基因突变

DOI:
10.3791/2355-v

•

07:25 min

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December 26, 2010

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, , , ,
1Department of Medical Microbiology and Immunology,University of California, Davis, 2Département Génétique et Développement, Institut Cochin,Université Paris Descartes

Chapters

  • 00:05Title
  • 01:19Transfection and Mutagenesis of Target Genes in Mosquito Cells
  • 04:14Post-transfection Cell Maintenance and Analysis
  • 05:46Representative Results for Transfection and Mutagenesis of Target Genes in Mosquito Cells by LNA-ONs
  • 07:00Conclusion

Summary

자동 번역

자동 번역

寡核苷酸，可用于网站，专门替代的转靶基因的单核苷酸<em>按蚊</em><em>按蚊</em>细胞。

Tags

TransfectionMutagenesisTarget GenesMosquito CellsLocked Nucleic Acid-modified OligonucleotidesPlasmodium ParasitesMalariaGenetically Modified MosquitoesCell Signaling PathwaysTargeted MutagenesisGene TargetsHomologous RecombinationDNA MoleculeStably Transformed Cell LinesLocked Nucleic Acid-modified Oligonucleotides (LNA-ONs)Artificial Single Nucleotide Substitutions

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