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Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
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Immunology and Infection
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JoVE Journal Immunology and Infection
Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

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10,287 Views

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07:25 min

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December 26, 2010

DOI:

10.3791/2355-v

DOI:
10.3791/2355-v

•

07:25 min
December 26, 2010

•

2 Views
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1Department of Medical Microbiology and Immunology,University of California, Davis, 2Département Génétique et Développement, Institut Cochin,Université Paris Descartes

Summary

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Oligonucleotides can be used to site specifically substitute a single nucleotide of transfected target genes in both Anopheles gambiae and Anopheles stephensi cells.

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TransfectionMutagenesisTarget GenesMosquito CellsLocked Nucleic Acid-modified OligonucleotidesPlasmodium ParasitesMalariaGenetically Modified MosquitoesCell Signaling PathwaysTargeted MutagenesisGene TargetsHomologous RecombinationDNA MoleculeStably Transformed Cell LinesLocked Nucleic Acid-modified Oligonucleotides (LNA-ONs)Artificial Single Nucleotide Substitutions

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Pakpour, N., Cheung, K. W., Souvannaseng, L., Concordet, J., Luckhart, S. Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides. J. Vis. Exp. (46), e2355, doi:10.3791/2355 (2010).

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