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Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
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Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
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December 26, 2010
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Summary
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Oligonucleotides can be used to site specifically substitute a single nucleotide of transfected target genes in both Anopheles gambiae and Anopheles stephensi cells.
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TransfectionMutagenesisTarget GenesMosquito CellsLocked Nucleic Acid-modified OligonucleotidesPlasmodium ParasitesMalariaGenetically Modified MosquitoesCell Signaling PathwaysTargeted MutagenesisGene TargetsHomologous RecombinationDNA MoleculeStably Transformed Cell LinesLocked Nucleic Acid-modified Oligonucleotides (LNA-ONs)Artificial Single Nucleotide Substitutions
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Pakpour, N., Cheung, K. W., Souvannaseng, L., Concordet, J., Luckhart, S. Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides. J. Vis. Exp. (46), e2355, doi:10.3791/2355 (2010).
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