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JoVE Journal Immunology and Infection
Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
 

Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

Article DOI: 10.3791/2355-v • 07:25 min • December 26th, 2010
December 26th, 2010 •
1, 1, 1, 2, 1
1Department of Medical Microbiology and Immunology, University of California, Davis, 2Département Génétique et Développement, Institut Cochin, Université Paris Descartes

Capítulos

Resumo
Automatic Translation

Oligonucleotides can be used to site specifically substitute a single nucleotide of transfected target genes in both Anopheles gambiae and Anopheles stephensi cells.

Tags

Transfection Mutagenesis Target Genes Mosquito Cells Locked Nucleic Acid-modified Oligonucleotides Plasmodium Parasites Malaria Genetically Modified Mosquitoes Cell Signaling Pathways Targeted Mutagenesis Gene Targets Homologous Recombination DNA Molecule Stably Transformed Cell Lines Locked Nucleic Acid-modified Oligonucleotides (LNA-ONs) Artificial Single Nucleotide Substitutions
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