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CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach

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Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

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Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach

DOI:

10.3791/201480-v

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03:39 min

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March 01, 2024

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Russell Spencer-Smith^2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Tags

생화학 CRAF RAF Kinase RAS GTPases Live Cell Assay Protein-protein Interactions KRAS-driven Cancers Noonan Syndrome RAF Dimerization RAF Autoinhibition RAF Function Therapeutic Targeting

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