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CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
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Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay
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Biochimica
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JoVE Journal Biochimica
Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach

DOI:
10.3791/201480-v

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03:39 min

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March 01, 2024

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1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Tags

BiochimicaCRAFRAF KinaseRAS GTPasesLive Cell AssayProtein-protein InteractionsKRAS-driven CancersNoonan SyndromeRAF DimerizationRAF AutoinhibitionRAF FunctionTherapeutic Targeting

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