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CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
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Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay
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Biochemie
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JoVE Journal Biochemie
Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

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03:39 min

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March 01, 2024

DOI:

10.3791/201480-v

DOI:
10.3791/201480-v

•

03:39 min
March 01, 2024

•

1 Views
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

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Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay. J. Vis. Exp. (Pending Publication), e201480, doi:10.3791/201480 (2024).

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