'Dergi'
/
Biyokimya
/
CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
/
Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay
JoVE Journal
Biyokimya
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Biyokimya
Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach

DOI:
10.3791/201480-v

•

03:39 min

•

March 01, 2024

•

1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

'Etiketler'

BiyokimyaCRAFRAF KinaseRAS GTPasesLive Cell AssayProtein-protein InteractionsKRAS-driven CancersNoonan SyndromeRAF DimerizationRAF AutoinhibitionRAF FunctionTherapeutic Targeting

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

Read Article