Journal
/
유전학
/
Analyzing tRNA Dynamics Using AQRNA-seq
/
Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation

Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation

24 Views

•

03:11 min

•

February 02, 2024

DOI:

10.3791/201424-v

DOI:
10.3791/201424-v

•

03:11 min
February 02, 2024

•

2 Views
, ,
1Department of Biological Engineering,Massachusetts Institute of Technology, 2Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group

Explore More Videos

Post-transcriptional MethylationAlkB DemethylaseRNA Sequencing PreparationAlkB Reaction BufferRNA-cDNA Hybrid HydrolysisPhase SeparationPhenol-chloroform ExtractionGel ElectrophoresisEthidium Bromide StainingPCR Product VisualizationSodium Hydroxide NeutralizationAgarose Gel Preparation

Read Article

Cite this Article

Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation. J. Vis. Exp. (Pending Publication), e201424, doi:10.3791/201424 (2024).

Download .ris file

Copy

Share Video

.

Copy