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Analyzing tRNA Dynamics Using AQRNA-seq
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Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation
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JoVE Journal Genetik
Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation

Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation

Analyzing tRNA Dynamics Using AQRNA-seq

DOI:
10.3791/201424-v

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03:11 min

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February 02, 2024

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, ,
1Department of Biological Engineering,Massachusetts Institute of Technology, 2Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group

Tags

AQRNA-seqSmall RNA QuantificationSequencing Read CountsLinear RelationshipMethylation RNA ModificationsBioinformatics PipelineTRNA QuantificationMycobacterium Bovis BCGNutrient DeprivationResuscitationPrimer DimerFull-length ReadsRead MappingHigh-throughput Implementation

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Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation

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