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Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation
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Genetik
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JoVE Journal Genetik
Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation

Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation

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03:11 min

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February 02, 2024

DOI:

10.3791/201424-v

DOI:
10.3791/201424-v

•

03:11 min
February 02, 2024

•

2 Views
, ,
1Department of Biological Engineering,Massachusetts Institute of Technology, 2Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group

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Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation. J. Vis. Exp. (Pending Publication), e201424, doi:10.3791/201424 (2024).

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