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Journal
/
Genetics
/
Analyzing tRNA Dynamics Using AQRNA-seq
/
Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation
JoVE Journal
Genetics
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JoVE Journal
Genetics
Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation
Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation
Analyzing tRNA Dynamics Using AQRNA-seq
DOI:
10.3791/201424-v
•
03:11 min
•
February 02, 2024
•
Ruixi Chen
,
Daniel Yim
,
Peter C. Dedon
2
1
Department of Biological Engineering
,
Massachusetts Institute of Technology
,
2
Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group
Tags
AQRNA-seq
Small RNA Quantification
Sequencing Read Counts
Linear Relationship
Methylation RNA Modifications
Bioinformatics Pipeline
TRNA Quantification
Mycobacterium Bovis BCG
Nutrient Deprivation
Resuscitation
Primer Dimer
Full-length Reads
Read Mapping
High-throughput Implementation
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Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation
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