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Mapping Alzheimer’s Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Mapping Alzheimer’s Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration

Mapping Alzheimer’s Disease Variants to Their Target Genes Using Computational Analysis of Chromatin Configuration

DOI:
10.3791/60428-v

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04:41 min

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January 09, 2020

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, , ,
1Department of Genetics,University of North Carolina, 2Neuroscience Center,University of North Carolina, 3Thurston Arthritis Research Center,University of North Carolina, 4Department of Cell Biology and Physiology,University of North Carolina

Chapters

  • 00:05Title
  • 01:00Generation of a Granges Object for Credible SNPs and Positional Mapping
  • 01:53Developmental Expression Trajectories
  • 02:18Cell-type Expression Profiles and Gene Annotation Enrichment Analysis
  • 02:46Results: Determination and Characterization of Putative Target Genes of AD GWS Loci
  • 03:52Conclusion

Summary

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We present a protocol to identify functional implications of non-coding variants identified by genome-wide association studies (GWAS) using three-dimensional chromatin interactions.

Tags

Alzheimer’s DiseaseGWASChromatin ConfigurationComputational AnalysisTarget GenesSNPsHi-CGene ExpressionCell-type ExpressionGene Annotation Enrichment

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