Journal
/
发育生物学
/
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

11,314 Views

•

11:00 min

•

January 12, 2015

DOI:

10.3791/52181-v

DOI:
10.3791/52181-v

•

11:00 min
January 12, 2015

•

3 Views
, , , , , , , , , , , , ,
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University

Summary

Automatically generated

The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

Explore More Videos

Adipose-derived Stromal CellsASCsBone Marrow-derived Mesenchymal Stromal CellsBM-MSCsOsteogenesisStromal Vascular FractionSVFCritical-sized Calvarial Defects

Read Article

Cite this Article

Zielins, E. R., Tevlin, R., Hu, M. S., Chung, M. T., McArdle, A., Paik, K. J., Atashroo, D., Duldulao, C. R., Luan, A., Senarath-Yapa, K., Walmsley, G. G., Wearda, T., Longaker, M. T., Wan, D. C. Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis. J. Vis. Exp. (95), e52181, doi:10.3791/52181 (2015).

Download .ris file

Copy

Share Video

.

Copy