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Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells
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Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

10,348 Views

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09:29 min

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July 10, 2019

DOI:

10.3791/59561-v

DOI:
10.3791/59561-v

•

09:29 min
July 10, 2019

•

3 Views
, ,
1Center for Neuroscience and Regenerative Medicine,Uniformed Services University, 2Department of Anatomy, Physiology, and Genetics,Uniformed Services University, 3Graduate Program in Neuroscience,Uniformed Services University

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This protocol demonstrates methodologies for a mouse model of open-skull traumatic brain injury and transplantation of cultured human induced pluripotent stem cell-derived cells into the injury site. Behavioral and histologic tests of outcomes from these procedures are also described in brief.

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Cite this Article

Furmanski, O., Nieves, M. D., Doughty, M. L. Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells. J. Vis. Exp. (149), e59561, doi:10.3791/59561 (2019).

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