Journal
/
发育生物学
/
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model

Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model

6,520 Views

•

09:07 min

•

February 24, 2017

DOI:

10.3791/55120-v

DOI:
10.3791/55120-v

•

09:07 min
February 24, 2017

•

1 Views
, , , , , , , , ,
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery,Stanford University School of Medicine

Summary

Automatically generated

Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.

Explore More Videos

Adipose-derived Stromal CellsBMPR-IB+Calvarial DefectCraniofacial RegenerationIn Vivo AssayCell IsolationCell CultureCell SortingOsteogenic ActivityRegenerative BiologyRBC LysisSubcutaneous Fat

Read Article

Cite this Article

Marshall, C. D., Zielins, E. R., Brett, E. A., Blackshear, C. P., Hu, M. S., Leavitt, T., Barnes, L. A., Lorenz, H. P., Longaker, M. T., Wan, D. C. Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model. J. Vis. Exp. (120), e55120, doi:10.3791/55120 (2017).

Download .ris file

Copy

Share Video

.

Copy