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发育生物学
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In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

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06:21 min

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April 25, 2019

DOI:

10.3791/59359-v

DOI:
10.3791/59359-v

•

06:21 min
April 25, 2019

•

3 Views
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1Department of Life Science Frontiers, Center for iPS Cells Research and Application,Kyoto University, 2Department of Clinical Application, Center for iPS Cells Research and Application,Kyoto University

Summary

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We present here a protocol for the differentiation of human induced pluripotent stem cells into each somite derivative (myotome, sclerotome, dermatome, and syndetome) in chemically defined conditions, which has applications in future disease modeling and cell-based therapies in orthopedic surgery.

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Induced Pluripotent Stem CellsIPSCSomite DerivativesMyotomeDermatomeSclerotomeSyndetomeParaxial MesodermPresomitic MesodermDelta-like Protein 1Regenerative TherapyDisease ModelingMusculoskeletal DisorderHuman Embryogenesis

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Cite this Article

Nakajima, T., Sakurai, H., Ikeya, M. In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (146), e59359, doi:10.3791/59359 (2019).

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