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Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
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发育生物学
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JoVE 杂志 发育生物学
Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

9,334 Views

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08:30 min

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February 24, 2017

DOI:

10.3791/54934-v

DOI:
10.3791/54934-v

•

08:30 min
February 24, 2017

•

2 Views
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1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea

Summary

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Mechanical stress can induce the chondrogenic differentiation of stem cells, providing a potential therapeutic approach for the repair of impaired cartilage. We present a protocol to induce the chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). CG-induced upregulation of SOX9 results in the development of chondrogenic phenotypes.

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Jang, Y., Jung, H., Ju, J. H. Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity. J. Vis. Exp. (120), e54934, doi:10.3791/54934 (2017).

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