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Journal
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Biology
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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
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Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues
Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
DOI:
10.3791/200322-v
•
03:38 min
•
July 28, 2023
•
Lucas Stalder
,
Fabian Koechl
,
Kerstin Hahn
,
Marc Sultan
,
Megana K. Prasad
1
Roche Pharma Research and Early Development, Pharmaceutical Sciences
,
Roche Innovation Centre Basel
Tags
Keywords: Single Nuclei
Sucrose Gradient Cleanup
Nuclei Suspension
SCS
Centrifugation
Propidium Iodide Staining
Cell Counter
Single Nuclei RNA Sequencing
Gene Counts
UMI Counts
Mitochondrial Content
Cellular Proportions
Technical Variability
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