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Immunology and Infection
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从切除的肺组织中分离支气管上皮细胞用于生物样本库并建立分化的气液界面培养物
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
Please note that all translations are automatically generated.
Click here for the English version.
从切除的肺组织中分离支气管上皮细胞用于生物样本库并建立分化的气液界面培养物
DOI:
10.3791/65102-v
•
08:42 min
•
May 26, 2023
•
Dennis K. Ninaber
,
Anne M. van der Does
,
Pieter S. Hiemstra
1
PulmoScience Laboratory, Department of Pulmonology
,
Leiden University Medical Center
Chapters
00:04
Introduction
00:47
Isolation of Bronchial Epithelial Cells from Human Lung Tissue
02:17
Cryopreservation of Human Primary Bronchial Epithelial Cells (PBECs)
03:28
Thawing Cryopreserved PBECs and Growing Them for Culture on Inserts
04:23
Establishing an Air‐Liquid Interface Culture with Primary Bronchial Epithelial Cells (ALI‐PBEC)
06:03
Results: Characterization of ALI Cultures Using Cell‐Specific Marker Genes
07:41
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是一种可重复、经济实惠且稳健的方法,用于分离和扩增原代支气管上皮细胞以进行长期生物样本库,并通过在气液界面培养产生分化的上皮细胞。
Tags
Bronchial Epithelial Cells
Biobanking
Air-liquid Interface Cultures
Cell Isolation
Protease 14
KSFM
Cryopreservation
Trypsinization
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