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Aislar células epiteliales bronquiales del tejido pulmonar resecado para biobancos y establecer cultivos de interfaz aire-líquido bien diferenciados
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Chapters
- 00:04Introduction
- 00:47Isolation of Bronchial Epithelial Cells from Human Lung Tissue
- 02:17Cryopreservation of Human Primary Bronchial Epithelial Cells (PBECs)
- 03:28Thawing Cryopreserved PBECs and Growing Them for Culture on Inserts
- 04:23Establishing an Air‐Liquid Interface Culture with Primary Bronchial Epithelial Cells (ALI‐PBEC)
- 06:03Results: Characterization of ALI Cultures Using Cell‐Specific Marker Genes
- 07:41Conclusion
