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Isolare le cellule epiteliali bronchiali dal tessuto polmonare resecato per il biobanking e stabilire colture di interfaccia aria-liquido ben differenziate
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
DOI:

08:42 min

May 26, 2023

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Chapters

  • 00:04Introduction
  • 00:47Isolation of Bronchial Epithelial Cells from Human Lung Tissue
  • 02:17Cryopreservation of Human Primary Bronchial Epithelial Cells (PBECs)
  • 03:28Thawing Cryopreserved PBECs and Growing Them for Culture on Inserts
  • 04:23Establishing an Air‐Liquid Interface Culture with Primary Bronchial Epithelial Cells (ALI‐PBEC)
  • 06:03Results: Characterization of ALI Cultures Using Cell‐Specific Marker Genes
  • 07:41Conclusion

Summary

Automatic Translation

Presentato qui è un metodo riproducibile, economico e robusto per l'isolamento e l'espansione delle cellule epiteliali bronchiali primarie per il biobanking a lungo termine e la generazione di cellule epiteliali differenziate mediante coltura all'interfaccia aria-liquido.

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