Presented here is a reproducible, affordable, and robust method for the isolation and expansion of primary bronchial epithelial cells for long-term biobanking and the generation of differentiated epithelial cells by culture at the air-liquid interface.
Ninaber, D. K., van der Does, A. M., Hiemstra, P. S. Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures. J. Vis. Exp. (195), e65102, doi:10.3791/65102 (2023).