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P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
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Immunology and Infection
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JoVE Journal Immunology and Infection
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives

English

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10,339 Views

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10:26 min

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June 15, 2020

DOI:

10.3791/61069-v

DOI:
10.3791/61069-v

•

10:26 min
June 15, 2020

•

29 Views
, , , , , , , ,
1Department of Drug Delivery,Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy,Saarland University, 3Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research, 4Department of Internal Medicine V – Pulmonology, Allergology, Respiratory Intensive Care Medicine,Saarland University Hospital

Summary

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We describe a protocol for a three-dimensional co-culture model of infected airways, using CFBE41o- cells, THP-1 macrophages, and Pseudomonas aeruginosa, established at the air-liquid interface. This model provides a new platform to simultaneously test antibiotic efficacy, epithelial barrier function, and inflammatory markers.

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Montefusco-Pereira, C. V., Horstmann, J. C., Ebensen, T., Beisswenger, C., Bals, R., Guzmán, C. A., Schneider-Daum, N., Carvalho-Wodarz, C. d. S., Lehr, C. P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives. J. Vis. Exp. (160), e61069, doi:10.3791/61069 (2020).

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