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使用荧光激活细胞核分选的脂肪细胞特异性ATAC-Seq与脂肪组织
JoVE Journal
Genetics
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JoVE Journal Genetics
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
DOI:

11:11 min

March 17, 2023

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Chapters

  • 00:05Introduction
  • 00:51Nucleus Isolation
  • 02:15Nucleus Sorting and Tn5 Tagmentation
  • 04:08DNA Purification and PCR Amplification
  • 05:32Real-Time Quantitative PCR (qPCR) Test, Additional PCR Amplification, and Second DNA Purification
  • 06:14DNA Fragment Size Selection
  • 08:01Results: Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing
  • 10:19Conclusion

Summary

Automatic Translation

我们提出了一种用于转座酶可访问染色质的高通量测序(ATAC-seq)测定的方案,专门针对脂肪细胞,使用细胞核分选,从具有核荧光标记的转基因报告小鼠中分离的脂肪组织。

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