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Genetics
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使用荧光激活细胞核分选的脂肪细胞特异性ATAC-Seq与脂肪组织
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Genetics
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JoVE Journal
Genetics
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
Please note that all translations are automatically generated.
Click here for the English version.
使用荧光激活细胞核分选的脂肪细胞特异性ATAC-Seq与脂肪组织
DOI:
10.3791/65033-v
•
11:11 min
•
March 17, 2023
•
Kyungchan Kim
,
Solaema Taleb
,
Jisun So
,
Jamie Wann
,
Hyun Cheol Roh
1
Department of Biochemistry and Molecular Biology
,
Indiana University School of Medicine
Chapters
00:05
Introduction
00:51
Nucleus Isolation
02:15
Nucleus Sorting and Tn5 Tagmentation
04:08
DNA Purification and PCR Amplification
05:32
Real-Time Quantitative PCR (qPCR) Test, Additional PCR Amplification, and Second DNA Purification
06:14
DNA Fragment Size Selection
08:01
Results: Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing
10:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种用于转座酶可访问染色质的高通量测序(ATAC-seq)测定的方案,专门针对脂肪细胞,使用细胞核分选,从具有核荧光标记的转基因报告小鼠中分离的脂肪组织。
Tags
Adipocyte-specific ATAC-seq
Fluorescence-activated Nucleus Sorting
Gene Expression Regulatory Mechanisms
Nuclear Isolation
FACS
Tn5 Transposase
ATAC-sequencing
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