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Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
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Genetica
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JoVE Journal Genetica
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

1,917 Views

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11:11 min

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March 17, 2023

DOI:

10.3791/65033-v

DOI:
10.3791/65033-v

•

11:11 min
March 17, 2023

•

13 Views
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1Department of Biochemistry and Molecular Biology,Indiana University School of Medicine

Summary

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We present a protocol for assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) specifically on adipocytes using nucleus sorting with adipose tissues isolated from transgenic reporter mice with nuclear fluorescence labeling.

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Adipocyte-specific ATAC-seqFluorescence-activated Nucleus SortingGene Expression Regulatory MechanismsNuclear IsolationFACSTn5 TransposaseATAC-sequencing

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Cite this Article

Kim, K., Taleb, S., So, J., Wann, J., Cheol Roh, H. Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting. J. Vis. Exp. (193), e65033, doi:10.3791/65033 (2023).

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