Described here is a method for analyzing bacterial gene expression in animal tissues at a cellular level. This method provides a resource for studying the phenotypic diversity occurring within a bacterial population in response to the tissue environment during an infection.
Behera, R. K., Mlynek, K. D., Linz, M. S., Brinsmade, S. R. A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues. J. Vis. Exp. (144), e59055, doi:10.3791/59055 (2019).