Journal
/
Genetics
/
ATAC-seq adipocita-specifico con tessuti adiposi mediante selezione del nucleo attivato dalla fluorescenza
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
Please note that all translations are automatically generated. Click here for the English version.

ATAC-seq adipocita-specifico con tessuti adiposi mediante selezione del nucleo attivato dalla fluorescenza

italiano

Automatically Generated

1,917 Views

•

11:11 min

•

March 17, 2023

DOI:

10.3791/65033-v

DOI:
10.3791/65033-v

•

11:11 min
March 17, 2023

•

13 Views
, , , ,
1Department of Biochemistry and Molecular Biology,Indiana University School of Medicine

Summary

Automatically generated

Presentiamo un protocollo per il dosaggio della cromatina accessibile alla trasposasi con sequenziamento ad alto rendimento (ATAC-seq) specificamente sugli adipociti utilizzando la selezione del nucleo con tessuti adiposi isolati da topi reporter transgenici con marcatura a fluorescenza nucleare.

Explore More Videos

Adipocyte-specific ATAC-seqFluorescence-activated Nucleus SortingGene Expression Regulatory MechanismsNuclear IsolationFACSTn5 TransposaseATAC-sequencing

Read Article

Cite this Article

Kim, K., Taleb, S., So, J., Wann, J., Cheol Roh, H. Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting. J. Vis. Exp. (193), e65033, doi:10.3791/65033 (2023).

Download .ris file

Copy

Share Video

.

Copy