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用于追踪斑马鱼径向胶质细胞祖细胞不对称分裂期间缺口/δ内吞作用的抗体摄取测定
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JoVE Journal
Developmental Biology
Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
Please note that all translations are automatically generated.
Click here for the English version.
用于追踪斑马鱼径向胶质细胞祖细胞不对称分裂期间缺口/δ内吞作用的抗体摄取测定
DOI:
10.3791/65030-v
•
09:06 min
•
January 20, 2023
•
Xiang Zhao
2
,
Su Guo
3
1
Chan Zuckerberg Biohub
,
2
Department of Bioengineering and Therapeutic Sciences
,
University of California San Francisco
,
3
Programs in Human Genetics and Biological Sciences
,
University of California San Francisco
Chapters
00:04
Introduction
01:01
Preparation of Zebrafish Embryos
02:10
Preparation of Microinjection
03:51
Microinjection
06:02
Mounting and Time‐Lapse Live Imaging
07:32
Results: Imaging Anti‐Dld‐Atto‐647N Antibody Uptake in the Developing Zebrafish Brain
08:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项工作开发了一种抗体摄取测定法,用于在胚胎斑马鱼大脑的径向胶质细胞祖细胞分裂中成像谱系内Notch/DeltaD信号传导。
Tags
Antibody Uptake Assay
Notch/Delta Endocytosis
Asymmetric Division
Zebrafish Radial Glia Progenitors
Notch Signaling
Sulfate Decision
Patterning
Development
Metazoans
Anti-Dld Antibody
Anti-mouse IgG Atto-647N Antibody
Brain Ventricles
Embryonic Development
Microinjection
Fluorescence Microscopy
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