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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

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Developmental Biology

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JoVE Journal Developmental Biology

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

4,170 Views

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08:00 min

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June 16, 2021

DOI:

10.3791/62041-v

DOI:

10.3791/62041-v

•

08:00 min

June 16, 2021

•

3 Views

Natasja G. J. Costermans^1,2, Enrico M. Daldello^1,2,3, Ria J. Marathe^1,2, Marco Conti^1,2

¹Center for Reproductive Sciences,University of California at San Francisco, ²Department of Obstetrics Gynecology and Reproductive Sciences,University of California at San Francisco, ³Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS,Sorbonne Université

Summary

This protocol describes a reporter assay to study the regulation of mRNA translation in single oocytes during in vitro maturation.

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