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Niet-waterige isolatie en verrijking van glandulaire capitate gestalkte en sessiele trichomen van Cannabis sativa
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Genetics
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JoVE Journal Genetics
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
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Niet-waterige isolatie en verrijking van glandulaire capitate gestalkte en sessiele trichomen van Cannabis sativa

Nederlands

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1,676 Views

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08:18 min

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May 12, 2023

DOI:

10.3791/64798-v

DOI:
10.3791/64798-v

•

08:18 min
May 12, 2023

•

16 Views
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1Department of Vegetable Research,ARO-Volcani Center, 2Department of Vegetable Research,ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences,ARO- Volcani Center, 4Department of Fruit Science,ARO- Volcani Center

Summary

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Er wordt een protocol gepresenteerd voor de handige en high-throughput isolatie en verrijking van glandulaire capitate stalked en sessiele trichomen van Cannabis sativa. Het protocol is gebaseerd op een droge, niet-bufferextractie van trichomen met alleen vloeibare stikstof, droogijs en nylonzeven en is geschikt voor RNA-extractie en transcriptomische analyse.

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Cohen, S., Itkin, M., Faigenboim, A., Davidovich-Rikanati, R., Bar, E., Hasson, D., Shalev, N., Koltai, H., Sagee, O., Lewinsohn, E., Spitzer-Rimon, B., Schaffer, A. A. Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa. J. Vis. Exp. (195), e64798, doi:10.3791/64798 (2023).

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