A protocol is presented for the convenient and high-throughput isolation and enrichment of glandular capitate stalked and sessile trichomes from Cannabis sativa. The protocol is based on a dry, non-buffer extraction of trichomes using only liquid nitrogen, dry ice, and nylon sieves and is suitable for RNA extraction and transcriptomic analysis.
Cohen, S., Itkin, M., Faigenboim, A., Davidovich-Rikanati, R., Bar, E., Hasson, D., Shalev, N., Koltai, H., Sagee, O., Lewinsohn, E., Spitzer-Rimon, B., Schaffer, A. A. Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa. J. Vis. Exp. (195), e64798, doi:10.3791/64798 (2023).