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Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
JoVE Journal
Genetica
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JoVE Journal Genetica
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

1,676 Views

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08:18 min

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May 12, 2023

DOI:

10.3791/64798-v

DOI:
10.3791/64798-v

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08:18 min
May 12, 2023

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1Department of Vegetable Research,ARO-Volcani Center, 2Department of Vegetable Research,ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences,ARO- Volcani Center, 4Department of Fruit Science,ARO- Volcani Center

Summary

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A protocol is presented for the convenient and high-throughput isolation and enrichment of glandular capitate stalked and sessile trichomes from Cannabis sativa. The protocol is based on a dry, non-buffer extraction of trichomes using only liquid nitrogen, dry ice, and nylon sieves and is suitable for RNA extraction and transcriptomic analysis.

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Non-aqueous IsolationEnrichmentGlandular Capitate Stalked TrichomesGlandular Capitate Sessile TrichomesCannabis SativaHigh ThroughputHigh ConcentrationTrichome DensityDry IceLiquid NitrogenMesh SieveExtractionPlant MaterialPlant Processing

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Cohen, S., Itkin, M., Faigenboim, A., Davidovich-Rikanati, R., Bar, E., Hasson, D., Shalev, N., Koltai, H., Sagee, O., Lewinsohn, E., Spitzer-Rimon, B., Schaffer, A. A. Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa. J. Vis. Exp. (195), e64798, doi:10.3791/64798 (2023).

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