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JoVE Journal Genetics
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
 

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

Article DOI: 10.3791/64798-v • 08:18 min • May 12th, 2023
May 12th, 2023 •
1, 1, 1, 2, 2, 1, 3, 3, 4, 2, 3, 1
1Department of Vegetable Research, ARO-Volcani Center, 2Department of Vegetable Research, ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences, ARO- Volcani Center, 4Department of Fruit Science, ARO- Volcani Center

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A protocol is presented for the convenient and high-throughput isolation and enrichment of glandular capitate stalked and sessile trichomes from Cannabis sativa. The protocol is based on a dry, non-buffer extraction of trichomes using only liquid nitrogen, dry ice, and nylon sieves and is suitable for RNA extraction and transcriptomic analysis.

Tags

Genetics Glandular Capitate Stalked Trichomes Sessile Trichomes Cannabis Sativa Protocol Convenience High Throughput High Concentration High Degree Of Isolation Straightforwardness Trichome Density Microscope Inspection Nurit Shalev Volcani Center Dry Ice Flakes Plastic Container Strainer Glass Beaker Frozen Cannabis Sativa Inflorescences Screen Door Mosquito Net Rubber Bands Liquid Nitrogen Stainless Steel Container
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