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Mouse In Vivo Placental Alvo CRISPR Manipulação
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Genetics
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JoVE Journal Genetics
Mouse In Vivo Placental Targeted CRISPR Manipulation

Mouse In Vivo Placental Alvo CRISPR Manipulação

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1,976 Views

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07:39 min

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April 14, 2023

DOI:

10.3791/64760-v

DOI:
10.3791/64760-v

•

07:39 min
April 14, 2023

•

10 Views
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1Interdisciplinary Graduate Program in Genetics,University of Iowa, 2Department of Psychiatry, Carver College of Medicine,University of Iowa, 3Iowa Neuroscience Institute, Carver College of Medicine,University of Iowa, 4Hawk-IDDRC,University of Iowa

Summary

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Aqui descrevemos um método tempo-específico para manipular efetivamente vias críticas de desenvolvimento na placenta de camundongos in vivo. Isto é realizado através da injeção e eletroporação de plasmídeos CRISPR nas placentas de fêmeas grávidas no dia embrionário 12.5.

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Cite this Article

Carver, A. J., Taylor, R. J., Stevens, H. E. Mouse In Vivo Placental Targeted CRISPR Manipulation. J. Vis. Exp. (194), e64760, doi:10.3791/64760 (2023).

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