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Mouse In Vivo Placental Targeted CRISPR Manipulation
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JoVE Journal Genetica
Mouse In Vivo Placental Targeted CRISPR Manipulation

Mouse In Vivo Placental Targeted CRISPR Manipulation

1,976 Views

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07:39 min

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April 14, 2023

DOI:

10.3791/64760-v

DOI:
10.3791/64760-v

•

07:39 min
April 14, 2023

•

10 Views
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1Interdisciplinary Graduate Program in Genetics,University of Iowa, 2Department of Psychiatry, Carver College of Medicine,University of Iowa, 3Iowa Neuroscience Institute, Carver College of Medicine,University of Iowa, 4Hawk-IDDRC,University of Iowa

Summary

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Here we describe a time-specific method to effectively manipulate critical developmental pathways in the mouse placenta in vivo. This is performed through the injection and electroporation of CRISPR plasmids into the placentas of pregnant dams on embryonic day 12.5.

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MousePlacentaCRISPRGenetic ManipulationIn VivoTargetedMid-late PregnancyOverexpressionAnesthesiaLaparotomyUterine HornPlacental InjectionElectroporation

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Cite this Article

Carver, A. J., Taylor, R. J., Stevens, H. E. Mouse In Vivo Placental Targeted CRISPR Manipulation. J. Vis. Exp. (194), e64760, doi:10.3791/64760 (2023).

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