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JoVE Journal Genetics
Mouse In Vivo Placental Targeted CRISPR Manipulation
 

Mouse In Vivo Placental Targeted CRISPR Manipulation

Article DOI: 10.3791/64760-v • 07:39 min • April 14th, 2023
April 14th, 2023 •
1,2,3, 2,3, 1,2,3,4
1Interdisciplinary Graduate Program in Genetics, University of Iowa, 2Department of Psychiatry, Carver College of Medicine, University of Iowa, 3Iowa Neuroscience Institute, Carver College of Medicine, University of Iowa, 4Hawk-IDDRC, University of Iowa

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Here we describe a time-specific method to effectively manipulate critical developmental pathways in the mouse placenta in vivo. This is performed through the injection and electroporation of CRISPR plasmids into the placentas of pregnant dams on embryonic day 12.5.

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Mouse Placental Targeted CRISPR Manipulation Gene Functions Mid To Late Pregnancy Genetic Manipulations Overexpression Gene Expression Changes Technique Anesthesia Surgical Site Disinfection Uterine Laparotomy
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