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Mouse In Vivo Placental Targeted CRISPR Manipulation
JoVE Journal
Genetik
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JoVE Journal Genetik
Mouse In Vivo Placental Targeted CRISPR Manipulation

Mouse In Vivo Placental Targeted CRISPR Manipulation

1,954 Views

07:39 min

April 14, 2023

DOI:

07:39 min
April 14, 2023

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Özet

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Here we describe a time-specific method to effectively manipulate critical developmental pathways in the mouse placenta in vivo. This is performed through the injection and electroporation of CRISPR plasmids into the placentas of pregnant dams on embryonic day 12.5.

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