Journal
/
Genetics
/
小鼠 体内 胎盘靶向CRISPR操作
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Mouse In Vivo Placental Targeted CRISPR Manipulation
Please note that all translations are automatically generated. Click here for the English version.

小鼠 体内 胎盘靶向CRISPR操作

English

Automatically Generated

1,975 Views

•

07:39 min

•

April 14, 2023

DOI:

10.3791/64760-v

DOI:
10.3791/64760-v

•

07:39 min
April 14, 2023

•

10 Views
, ,
1Interdisciplinary Graduate Program in Genetics,University of Iowa, 2Department of Psychiatry, Carver College of Medicine,University of Iowa, 3Iowa Neuroscience Institute, Carver College of Medicine,University of Iowa, 4Hawk-IDDRC,University of Iowa

Summary

Automatically generated

在这里，我们描述了一种时间特异性方法，以有效操纵 体内小鼠胎盘中的关键发育途径。这是通过在胚胎第12.5天将CRISPR质粒注射和电穿孔到妊娠母亲的胎盘中进行的。

Explore More Videos

MousePlacentaCRISPRGenetic ManipulationIn VivoTargetedMid-late PregnancyOverexpressionAnesthesiaLaparotomyUterine HornPlacental InjectionElectroporation

Read Article

Cite this Article

Carver, A. J., Taylor, R. J., Stevens, H. E. Mouse In Vivo Placental Targeted CRISPR Manipulation. J. Vis. Exp. (194), e64760, doi:10.3791/64760 (2023).

Download .ris file

Copy

Share Video

.

Copy