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筛选精子以快速分离斑马鱼的种系编辑

JoVE Journal
Genetics

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JoVE Journal Genetics

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

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筛选精子以快速分离斑马鱼的种系编辑

1,059 Views

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05:55 min

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February 10, 2023

DOI:

10.3791/64686-v

DOI:

10.3791/64686-v

•

05:55 min

February 10, 2023

•

3 Views

Brittney Voigt¹, Ryoko Minowa², Ryan S. Gray^1,2

¹Department of Molecular Biosciences,The University of Texas at Austin, ²Department of Nutritional Sciences,The University of Texas at Austin

Summary

CRISPR-Cas技术彻底改变了基因组编辑领域。然而，寻找和分离所需的种系编辑仍然是一个主要瓶颈。因此，该协议描述了一种使用标准PCR，限制性酶切和凝胶电泳技术快速筛选F0 CRISPR注射斑马鱼精子以进行种系编辑的稳健方法。

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