Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Brittney Voigt; Ryoko Minowa; Ryan S. Gray

doi:10.3791/64686

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遺伝学

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Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

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遺伝学

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JoVE Journal 遺伝学

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

1,062 Views

•

05:55 min

•

February 10, 2023

DOI:

10.3791/64686-v

DOI:

10.3791/64686-v

•

05:55 min

February 10, 2023

•

3 Views

Brittney Voigt¹, Ryoko Minowa², Ryan S. Gray^1,2

¹Department of Molecular Biosciences,The University of Texas at Austin, ²Department of Nutritional Sciences,The University of Texas at Austin

概要

CRISPR-Cas technologies have revolutionized the field of genome editing. However, finding and isolating the desired germline edit remains a major bottleneck. Therefore, this protocol describes a robust method for quickly screening F0 CRISPR-injected zebrafish sperm for germline edits using standard PCR, restriction digest, and gel electrophoresis techniques.

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Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

概要

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